RESUMO
Abstract INTRODUCTION Salmonella enterica serovar Panama belongs to the D1 serogroup and is frequently associated with nontyphoidal salmonellosis in humans. This study aimed to characterize isolates collected from Northeast Brazil by phenotypic and molecular methods. METHODS Forty four S. Panama strains were examined for antimicrobial susceptibility, virulence genes, and pulsed field gel electrophoresis (PFGE) types. RESULTS All strains were susceptible to antibiotics (except for streptomycin), presented classical virulence factors, and could be clustered into four groups and 18 pulsotypes. CONCLUSIONS This work calls for continuous surveillance for the emergence of antibiotic resistance and new clones in a geographical area.
Assuntos
Animais , Salmonella enterica/genética , Fatores de Virulência/genética , Variação Genética , Brasil , Testes de Sensibilidade Microbiana , Eletroforese em Gel de Campo Pulsado , Salmonella enterica/isolamento & purificação , Salmonella enterica/efeitos dos fármacos , Salmonella enterica/patogenicidade , Farmacorresistência Bacteriana Múltipla , Antibacterianos/farmacologiaRESUMO
O objetivo deste experimento foi produzir uma infecção experimental de Salmonella enterica subespécie. enterica sorotipo Panama e verificar a importância da via nasonasal na transmissão entre leitões desmamados. Foram utilizados seis leitões recém-desmamados, adquiridos de granja livre de Salmonella spp. Utilizaram-se baias isoladoras, que proporcionavam o contato nasonasal e eliminavam a possibilidade de outras vias de transmissão e de contaminação externa. Três grupos foram formados: controle, sentinela e infectado. Não foram encontradas amostras positivas para Salmonella spp. em leitões do grupo-controle e sentinelas, e nos animais infectados foi isolada Salmonella Panama em suabes retais e tecidos necropsiados. Os resultados revelaram não haver a transmissão pela via nasonasal entre leitões desmamados, pois, em nenhum momento, o agente foi isolado dos animais sentinelas.
Experimental infection of Salmonella enterica ssp. enterica serovars Panama was proceeded to establish the importance of nose-to-nose contact in the transmission among weaned pigs. Six recently-weaned pigs were acquired from a farm previously selected, free from Salmonella spp. Isolation stalls allowing nose-to-nose contact and eliminating other transmission routes as well outside contamination were used. Three groups were formed: control, sentinel, and infected. Piglets of control and sentinel groups were not positive for Salmonella spp., however, Salmonella Panama was isolated in infected animals in rectal swabs and necropsied tissues. It was concluded that there was not transmission by nose-to-nose contact among weaned pigs, since Samonella Panama was not isolated in the sentinel group at any moment.
